Reagent | Final concentration or percentage | Amount |
---|---|---|
Tris base (1 M, pH 7.4) | 20 mM | 50 mL |
NaCl (5 M) | 140 mM | 30 mL |
Tween-20 | 0.1% | 1 mL |
MilliQ water | n/a | 919 mL |
Total | n/a | 1 L |
Reagent | Final concentration or percentage | Amount |
---|---|---|
Tris base (1 M, pH 7.4) | 20 mM | 50 mL |
NaCl (5 M) | 140 mM | 30 mL |
Tween-20 | 0.1% | 1 mL |
MilliQ water | n/a | 919 mL |
Total | n/a | 1 L |