15% acrylamide gel solution

Reagent	Final concentration or percentage	Amount
MilliQ water	n/a	1.250 mL
30% acrylamide	15%	2.75 mL
Tris (1.5 M, pH 8.8)	375 mM	1.375 mL
SDS (10%)	1%	55 μL
APS (10%)	1.18%	65 μL
TEMED	0.09%	5 μL
Total	n/a	5.5 mL