Acrylamide stack solution
| Reagent | Final concentration or percentage | Amount |
|---|---|---|
| MilliQ water | n/a | 1.37 mL |
| 30% acrylamide | 5% | 335 μL |
| Tris (1.0 M, pH 6.8) | 125 mM | 250 μL |
| SDS (10%) | 1.0% | 20 μL |
| APS (10%) | 1.0% | 20 μL |
| TEMED | 0.25% | 5 μL |
| Total | n/a | 2 mL |
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