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1× RIPA buffer
| Reagent | Final concentration or percentage | Amount |
|---|---|---|
| MilliQ water | n/a | 720 μL |
| 10× RIPA buffer | 1× | 100 μL |
| DTT (100 mM) | 1 mM | 10 μL |
| PMSF (100 mM) | 1 mM | 10 μL |
| Protease Inhibitor Cocktail | 15% | 150 μL |
| Phosphatase Inhibitor Cocktail | 1% | 10 μL∗ |
| Total | n/a | 1 mL |
