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. 2021 Jan 5;19:577–585. doi: 10.1016/j.csbj.2020.12.043

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© 2021 The Authors. Published by Elsevier B.V. on behalf of Research Network of Computational and Structural Biotechnology.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 5 — Comparison of enzymatic properties and structure visualization of positive combinatorial variants. (A) Specific activity. (B) Apparent melting temperature curve detected via the differential scanning fluorimetry method. (C) The first-order deactivation curve measured under standard conditions. (D) Structure visualization of T73(O2beY)-171C and a positive combinatorial variant. The green dashed line refers to the centroid distance between the two aromatic rings. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)