Figure 1.

Venn diagram of proteins adsorbed on titanium (Ti), enamel, and dentine surfaces from saliva (S) and plasma (P)

Surfaces were exposed to human stimulated saliva or human plasma for 2 h at 35°C in an orbital shaker (70 rpm) and evaluated by liquid chromatography coupled with tandem mass spectrometry (LC–MS/MS). Numbers in parentheses are total proteins identified in each surface. Although there are shared proteins among the surfaces and in the different fluids (saliva and plasma), all groups/surfaces showed unique proteins adsorbed, showing the effect of chemical and physical properties of substrate to modulate protein adsorption.