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. 2020 Dec 29;24(1):102003. doi: 10.1016/j.isci.2020.102003

Figure 5.

Figure 5

KRT8/Krt8 is expressed and essential in mouse primordial germ cells (PGCs) and human primordial germ cell-like cells (hPGCLCs) migration

(A) Immunohistochemistry staining of Ddx4 and Krt8 and the quantification of Ddx4-expressing (Ddx4+) and Krt8-expressing (Krt8+) cells at different days of mouse embryos.

(B) KRT8 knockdown (KRT8-KD) cells were generated using siRNA transfection and KRT8 mRNA levels were analyzed by qRT-PCR in hPGCLCs. Representative immunofluorescent images of filters stained with DDX4 and KRT8 in a trans-well cell migration assay. KRT8-KD or siControl cells were plated in the upper chamber and the migration was stimulated by the addition of 10% FBS. The migratory cells were also stained with hematoxylin-eosin (right panels). Scale bar, 100um.

(C) The migratory cells were quantified by counting the DDX4+ and KRT8+ cells in several random fields.

Mann-Whitney U test was used for statistical analysis. Bar graph represents mean ± SEM from at least three independent experiments. ∗p < 0.05, ∗∗∗p < 0.001.