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. 2021 Jan 13;2(1):100261. doi: 10.1016/j.xpro.2020.100261

Figure 3.

Figure 3

Characterization of hiPSC-derived pericytes from WTC11, WT83, Q83X, and M2 hiPSC cell lines

(A) Flow cytometry of pericytes for pericyte markers CD13 and CD140b.

(B) Representative immunofluorescent images of hiPSC-derived pericytes stained positively for markers SM22 (red) and αSMA (green) and negatively for the endothelial marker CD31 (purple). Nuclei are counterstained with DAPI (blue). Scale bar represents 100 μm.

(C and D) Representative immunofluorescent images of hiPSC (WTC11)-derived endothelial cells (C) and hiPSC (WTC11)-derived endothelial cells and hiPSC (WTC11)-derived pericytes cocultured (D) at day 5 of an angiogenesis assay. hiPSC-derived endothelial cells stained positively for the endothelial marked CD31 (C, purple) (D, green) and hiPSC-derived pericytes stained positively for the pericyte markers SM22 (C, green), collagen IV (C, red) and PDGFRβ (D, red). Nuclei are counterstained with DAPI (blue). Scale bars represent 500 μm and 50 μm.