Table 1.
Antibodies for immunofluorescence
| Antibody | Dilution | Supplier |
|---|---|---|
| Conjugated antibody | ||
| PE Mouse anti-human CD31 | 1:5 | BD Pharmingen |
| PE Mouse anti-human CD140b | 1:5 | BD Pharmingen |
| Primary antibody | ||
| Anti-α-Smooth Muscle Acting (1A4) monoclonal mouse | 1:200 | Cell Signaling Technology |
| Anti-CD31 monoclonal hamster | 1:250 | Abcam |
| Anti-TAGLN (SM22) polyclonal rabbit | 1:200 | Thermo Fisher |
| Anti-collagen IV monoclonal mouse | 1:62.5 | R&D Systems |
| Anti-PDGFR beta monoclonal rabbit | 1:100 | Abcam |
| Secondary antibody | ||
| Alex Fluor 488 donkey anti-mouse | 1:200 | Life Technologies |
| Alexa Fluor 568 donkey anti-rabbit | 1:200 | Life Technologies |
| Alexa Fluor 647 goat anti-hamster | 1:200 | Life Technologies |