Antibodies |
|
PE mouse anti-human CD13 |
BD Pharmingen |
555394 |
PE mouse anti-human CD140b |
BD Pharmingen |
558821 |
Anti-α-smooth muscle actin (1A4) mouse mAb |
Cell Signaling Technology |
48938 |
Anti-CD31 hamster mAb |
Abcam |
ab119341 |
Anti-TAGLN (SM22) rabbit polyclonal antibody |
Thermo Fisher |
PA5-27463 |
Alexa Fluor 488 donkey anti-mouse IgG |
Life Technologies |
A21202 |
Alexa Fluor 568 donkey anti-rabbit IgG |
Life Technologies |
A10042 |
Alex Fluor 657 goat anti-hamster IgG |
Life Technologies |
A21451 |
|
Chemicals, peptides, and recombinant proteins |
|
Accutase |
Corning |
25-058-CI |
Essential 8 Medium |
Thermo Fisher |
A1517001 |
Versene |
Life Technologies |
15040-066 |
Matrigel |
Corning |
354277 |
Geltrex Matrigel |
Gibco |
A14133-02 |
Tocris Bioscience DAPI |
Fisher Scientific |
5748 |
PBS |
Fisher Scientific |
1419144 |
EDTA |
Invitrogen |
130-036-702 |
Fibronectin |
Corning |
356008 |
Collagen IV |
Corning |
354245 |
EGM-2 Medium Kit |
Promocell |
C-22111 |
TrypLE |
Life Technologies |
12563-029 |
PM Media Kit |
ScienCell |
1201,1252,0010 |
FBS |
Gibco |
16000044 |
Normal Donkey Serum |
Fisher Scientific |
50-413-115 |
DMSO |
Fisher Scientific |
BP231 |
BMP4 |
R&D Systems |
314-BP-010/CF |
Activin A |
R&D Systems |
338-AC-50/CF |
CHIR 99021 |
R&D Systems |
4423 |
DF3S medium |
Gibco |
A14535DJ |
SB431542 |
R&D Systems |
1614 |
VEGFA165 |
R&D Systems |
293-VE-500/CF |
FGF2 |
R&D Systems |
233-FB-500/CF |
Transferrin |
R&D Systems |
2914-HT |
Insulin |
Sigma |
I9278 |
Paraformaldehyde |
Fisher Scientific |
18612139 |
Rock Inhibitor Y27632 |
R&D Systems |
1254/10 |
|
Critical commercial assays |
|
Miltenyi Biotech MACS 34 microbead kit |
Miltenyi Biotech |
130046702 |
MACS LS Column |
Miltenyi Biotech |
130042401 |
MACS MS Column |
Miltenyi Biotech |
130042201 |
|
Experimental models: cell lines |
|
iPSC line WTC-11 (GM25256) |
Coriell Institute |
GM25256 |
iPSC line WT-83 |
University of California San Diego |
N/A |
iPSC line Q83X |
University of California San Diego |
N/A |
iPSC line M2 |
University of California San Diego |
N/A |
Human Brain Vascular Pericytes |
ScienCell |
1200 |
|
Other |
|
6-well tissue culture plate |
Corning |
3516 |
100 mm tissue culture dish |
Corning |
353003 |
Angiogenesis Plate |
Ibidi |
89646 |