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. 2021 Jan 13;2(1):100261. doi: 10.1016/j.xpro.2020.100261
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

PE mouse anti-human CD13 BD Pharmingen 555394
PE mouse anti-human CD140b BD Pharmingen 558821
Anti-α-smooth muscle actin (1A4) mouse mAb Cell Signaling Technology 48938
Anti-CD31 hamster mAb Abcam ab119341
Anti-TAGLN (SM22) rabbit polyclonal antibody Thermo Fisher PA5-27463
Alexa Fluor 488 donkey anti-mouse IgG Life Technologies A21202
Alexa Fluor 568 donkey anti-rabbit IgG Life Technologies A10042
Alex Fluor 657 goat anti-hamster IgG Life Technologies A21451

Chemicals, peptides, and recombinant proteins

Accutase Corning 25-058-CI
Essential 8 Medium Thermo Fisher A1517001
Versene Life Technologies 15040-066
Matrigel Corning 354277
Geltrex Matrigel Gibco A14133-02
Tocris Bioscience DAPI Fisher Scientific 5748
PBS Fisher Scientific 1419144
EDTA Invitrogen 130-036-702
Fibronectin Corning 356008
Collagen IV Corning 354245
EGM-2 Medium Kit Promocell C-22111
TrypLE Life Technologies 12563-029
PM Media Kit ScienCell 1201,1252,0010
FBS Gibco 16000044
Normal Donkey Serum Fisher Scientific 50-413-115
DMSO Fisher Scientific BP231
BMP4 R&D Systems 314-BP-010/CF
Activin A R&D Systems 338-AC-50/CF
CHIR 99021 R&D Systems 4423
DF3S medium Gibco A14535DJ
SB431542 R&D Systems 1614
VEGFA165 R&D Systems 293-VE-500/CF
FGF2 R&D Systems 233-FB-500/CF
Transferrin R&D Systems 2914-HT
Insulin Sigma I9278
Paraformaldehyde Fisher Scientific 18612139
Rock Inhibitor Y27632 R&D Systems 1254/10

Critical commercial assays

Miltenyi Biotech MACS 34 microbead kit Miltenyi Biotech 130046702
MACS LS Column Miltenyi Biotech 130042401
MACS MS Column Miltenyi Biotech 130042201

Experimental models: cell lines

iPSC line WTC-11 (GM25256) Coriell Institute GM25256
iPSC line WT-83 University of California San Diego N/A
iPSC line Q83X University of California San Diego N/A
iPSC line M2 University of California San Diego N/A
Human Brain Vascular Pericytes ScienCell 1200

Other

6-well tissue culture plate Corning 3516
100 mm tissue culture dish Corning 353003
Angiogenesis Plate Ibidi 89646