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E8BAC medium
| Reagent | Final concentration | Volume |
|---|---|---|
| Essential 8 Media | n/a | 50 mL |
| E8 Supplement | 2% | 1 mL |
| BMP4 | 5 ng/mL | 2.5 μL |
| Activin A | 25 ng/mL | 12.5 μL |
| CHIR 99021 | 1 μM | 5 μL |
When plating cells from thaw or passaging, add Rock inhibitor Y27632 to media for a final concentration of 10 μM for the initial 24 h.
Store at 4°C for up to 2 weeks.
