Effect of 7,8-DHF on food intake in the LFD- and HFD-fed mice. (A) Average food intake (g/day) per mouse fed the LFD (low-fat diet) and LFD+DHF10 each week. (B) Average food intake (g/day) per mouse fed the HFD (high-fat diet), HFD+DHF5, HFD+DHF10, and HFD+DHF20 each week. (C) Daily food intake was measured per cage per mouse after treatment with 7,8-DHF for 24 weeks (n = 3 cages per group; food intake was monitored by weighting daily for 7 days in each cage of each group and then calculated as the average value per cage per mouse, 3 cages per group and 4 mice per cage, ∗p < 0.05, LFD control vs LFD+DHF10, Student’s t test; N.S: not significant, HFD control vs HFD+DHF5, HFD+DHF10, and HFD+DHF20, one-way ANOVA).