7,8-DHF reduced diet-induced obesity and increased bone mineral density in mature adult female mice. (A) Quantitative analysis of total fat volume (TFV), subcutaneous fat volume (SFV), and visceral fat volume (VFV) by micro-CT (representative coronal and transverse sections from the same experiment; visceral, red; subcutaneous, yellow). (B) Bone mineral density measured by micro-CT. Scan regions of the total body bone density (TBD) are represented in red, spine bone density (SBD) in yellow, and femoral bone density (FBD) in green (n = 3 mice per group; N.S: not significant, ∗∗p < 0.01, LFD control vs LFD+DHF10, Student's t test; N.S: not significant, ##p < 0.01, ###p < 0.001, HFD control vs LFD control, Student's t test; &p < 0.05, &&p < 0.01, HFD control vs HF+DHF5, HFD+DHF10, and HFD+DHF20, one-way ANOVA).