. 2020 Dec 19;45:101149. doi: 10.1016/j.molmet.2020.101149

Table 1.

Serum and tissue parameters related to the hypothalamic-pituitary-ovarian (HPO) axis function in mice after treatment with 7,8-DHF for 24 weeks.

Indexes		Groups
		LFD-fed mice		HFD-fed mice
		LFD control	LFD + DHF₁₀	HFD control	HFD + DHF₅	HFD + DHF₁₀	HFD + DHF₂₀
Estrus frequency (times/week)		0.88 ± 0.15	1.56 ± 0.14∗∗∗	0.81 ± 0.19^N.S	1.52 ± 0.19^&&&	1.55 ± 0.12^&&&	1.48 ± 0.13^&&&
Serum (n = 10–12)	E2 (pg/mL)	36.00 ± 2.85	51.31 ± 9.68∗∗∗	37.53 ± 4.23^N.S	56.30 ± 8.20^&&&	56.06 ± 6.61^&&&	52.36 ± 7.99^&&&
	FSH (mIU/mL)	12.46 ± 2.13	6.72 ± 1.14∗∗∗	15.28 ± 3.02^#	7.79 ± 1.43^&&&	7.24 ± 1.22^&&&	8.19 ± 1.52^&&&
	AMH (ng/mL)	3.30 ± 0.60	4.55 ± 0.63∗∗∗	2.89 ± 0.56^N.S	4.49 ± 0.69^&&&	4.65 ± 0.62^&&&	4.35 ± 0.68^&&&
Uterus (n = 10–12)	Uterus weight (mg)	64.66 ± 15.71	79.21 ± 13.03∗	79.15 ± 12.77^#	82.20 ± 10.62^N	87.20 ± 14.46^N	82.59 ± 14.13^N
Uterus (n = 10–12)	Uterus index (%)	0.32 ± 0.06	0.38 ± 0.05∗	0.23 ± 0.05^###	0.28 ± 0.04^N	0.29 ± 0.05^&	0.26 ± 0.04^N
Ovary (n = 3–4)	Pr (counts)	286.3 ± 35.2	685.0 ± 66.8∗∗∗	76.3 ± 8.0^###	253.3 ± 33.2^&&&	–	–
	P (counts)	31.7 ± 2.4	68.3 ± 6.2∗∗	6.5 ± 2.1^###	58.3 ± 14.3^&&&	–	–
	S (counts)	43.7 ± 6.3	88.7 ± 5.8∗∗	35.5 ± 7.3^N.S	96.7 ± 10.3^&&&	–	–
	A (counts)	46.3 ± 1.9	52.0 ± 5.2^n.s	32.5 ± 8.3^#	88.3 ± 8.5^&&&	–	–
	Af (counts)	2196.7 ± 248.7	1293.3 ± 149.7∗∗∗	2915.5 ± 309.3^#	2076.7 ± 241.4^&	–	–

Pr: primordial follicles; P: primary follicles; S: secondary follicles; A: healthy antral follicles; Af: atretic follicles. The uterus index was calculated as uterus weight divided by body weight. N.S: not significant, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 vs the LFD control group, Student's t test; N: not significant, ^&p < 0.05, ^&&&p < 0.001 vs the HFD control group, one-way ANOVA; N.S: not significant, ^#p < 0.05, ^###p < 0.001 vs the LFD control group, Student's t test; “–” represents not tested.