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. 2020 Dec 24;45:101157. doi: 10.1016/j.molmet.2020.101157

Figure 3.

Figure 3

Loss of dystrophin and utrophin in dystrophic muscles impairs mitochondrial remodeling to low-frequency stimulation (LFS). (A) Expression of respiratory chain complexes assessed by mitochondrial proteomics in gastrocnemius (GAS) muscles (n = 3/group). (B) Representative image of complex I enzyme activity (native and supercomplexes) and total protein staining (Coomassie) assessed by blue native polyacrylamide gel electrophoresis (PAGE) in GAS muscles. (C) Percentage of mitochondrial complex I in native and supercomplexes assessed by blue native PAGE in GAS muscles (n = 6/group). (D) Representative image of Sirt1 and Sirt3 protein expression assessed by western blot in whole GAS muscle lysate. (E and F) Quantification of Sirt1 (E) and Sirt3 (F) protein expression assessed by western blot in whole GAS muscle lysate (n = 6/group). Data are means ± SEM; unpaired Student's t-test (C); two-way ANOVA followed by Tukey's multiple comparison test (E and F); ME: main effect; † difference to BL/10; ‡ difference to mdx; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗∗p < 0.0001.