Figure 5.
Obtaining isolated mouse mammary epithelial cells by filtration
(A) Schematic of homemade filter device.
(B) The end of the plastic syringe cap is used to clamp the nylon mesh (70 μm pore) to the syringe. The syringe needle has been discarded. The cap can be soaked in 70% ethanol after cutting and dried before proceeding to the next step.
(C) Physical assembly of the sterile cell syringe filter.