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. 2021 Jan 13;2(1):100270. doi: 10.1016/j.xpro.2020.100270
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Biotin anti-CD140 BioLegend RRID:AB_11211998
Biotin anti-CD31 BioLegend RRID:AB_312910
Biotin anti-Ter-119 BioLegend RRID:AB_313704
Biotin anti-CD45 BioLegend RRID:AB_312968
Anti-Sca1-PerCP BioLegend RRID:AB_893618
Anti-CD49b-APC/Cy7 BioLegend RRID:AB_313416
Anti-EpCAM-APC BioLegend RRID:AB_1134105
Anti-CD49f-PE/Cy7 BioLegend RRID:AB_2561704
Rat IgG isotype control Invitrogen RRID:AB_2532969

Chemicals, peptides, and recombinant proteins

Accumax Millipore Sigma SCR006
Collagenase A Millipore Sigma 11088793001
100× penicillin/streptomycin Thermo Fisher 15140122
DNase Millipore Sigma D4527-200KU
10× Trypsin Thermo Fisher 15400054
DMEM/F12 Thermo Fisher 11330032
Brilliant Violet 510 Streptavidin BioLegend 405233
Cell Trace Violet Life Technologies C34557
Zombie Yellow BioLegend 423104
Fetal bovine serum R&D Systems Cat # S11150
Gibco PBS, pH 7.4 Thermo Fisher Scientific Cat # 10010023

Experimental models: organisms/strains

Mouse: C57BL/6JRSK2−/−(RSK2-KO) age range 6–12 weeks Institut de Genetique et Biologie Moleculaire et Cellulaire, C.U. de Strasbourg, France Andre Hanauer, PhD

Software and algorithms

LSM-FCS/ZEN Carl Zeiss N/A
GraphPad Prism 6.0a GraphPad N/A
BioRender BioRender https://biorender.com/
Cytobank Cytobank https://cytobank.org/

Other

Becton-Dickinson’s FACAria II BD Biosciences n/a
Eppendorf New Brunswick Galaxy 170R CO2 incubator VWR n/a
Eppendorf ThermoMixer VWR n/a
Thermo/IEC Centra CL2 benchtop centrifuge Thermo Scientific n/a
236 Aerocarrier rotor Thermo Scientific n/a
Aerocarrier inserts for 236 Aerocarrier rotor for 2 × 15 mL Falcon/Corning Conical Thermo Scientific n/a
Eppendorf centrifuge 5424R Fisher Scientific n/a
Rotor FA-45-24-11 Fisher Scientific n/a
Hausser Scientific Levy hemacytometer Fisher Scientific Cat # 02-671-55A
WPI Iris scissors, 10 cm, supercut, straight Fisher Scientific Cat # 50-822-291
Micro dissecting forceps serrated, half curved 1 mm tip, 4 inch George Tiemann Cat # 160-18
Syringe filters, sterile Fisher Scientific Cat # 09720004
70 μm nylon mesh Fisher Scientific Cat # NC0446099

Deposited data

RNA sequencing data Ludwik et al. 2020 GEO:GSE113323