Antibodies |
|
Biotin anti-CD140 |
BioLegend |
RRID:AB_11211998
|
Biotin anti-CD31 |
BioLegend |
RRID:AB_312910
|
Biotin anti-Ter-119 |
BioLegend |
RRID:AB_313704
|
Biotin anti-CD45 |
BioLegend |
RRID:AB_312968
|
Anti-Sca1-PerCP |
BioLegend |
RRID:AB_893618
|
Anti-CD49b-APC/Cy7 |
BioLegend |
RRID:AB_313416
|
Anti-EpCAM-APC |
BioLegend |
RRID:AB_1134105
|
Anti-CD49f-PE/Cy7 |
BioLegend |
RRID:AB_2561704
|
Rat IgG isotype control |
Invitrogen |
RRID:AB_2532969
|
|
Chemicals, peptides, and recombinant proteins |
|
Accumax |
Millipore Sigma |
SCR006 |
Collagenase A |
Millipore Sigma |
11088793001 |
100× penicillin/streptomycin |
Thermo Fisher |
15140122 |
DNase |
Millipore Sigma |
D4527-200KU |
10× Trypsin |
Thermo Fisher |
15400054 |
DMEM/F12 |
Thermo Fisher |
11330032 |
Brilliant Violet 510 Streptavidin |
BioLegend |
405233 |
Cell Trace Violet |
Life Technologies |
C34557 |
Zombie Yellow |
BioLegend |
423104 |
Fetal bovine serum |
R&D Systems |
Cat # S11150
|
Gibco PBS, pH 7.4 |
Thermo Fisher Scientific |
Cat # 10010023 |
|
Experimental models: organisms/strains |
|
Mouse: C57BL/6JRSK2−/−(RSK2-KO) age range 6–12 weeks |
Institut de Genetique et Biologie Moleculaire et Cellulaire, C.U. de Strasbourg, France |
Andre Hanauer, PhD |
|
Software and algorithms |
|
LSM-FCS/ZEN |
Carl Zeiss |
N/A |
GraphPad Prism 6.0a |
GraphPad |
N/A |
BioRender |
BioRender |
https://biorender.com/ |
Cytobank |
Cytobank |
https://cytobank.org/ |
|
Other |
|
Becton-Dickinson’s FACAria II |
BD Biosciences |
n/a |
Eppendorf New Brunswick Galaxy 170R CO2 incubator |
VWR |
n/a |
Eppendorf ThermoMixer |
VWR |
n/a |
Thermo/IEC Centra CL2 benchtop centrifuge |
Thermo Scientific |
n/a |
236 Aerocarrier rotor |
Thermo Scientific |
n/a |
Aerocarrier inserts for 236 Aerocarrier rotor for 2 × 15 mL Falcon/Corning Conical |
Thermo Scientific |
n/a |
Eppendorf centrifuge 5424R |
Fisher Scientific |
n/a |
Rotor FA-45-24-11 |
Fisher Scientific |
n/a |
Hausser Scientific Levy hemacytometer |
Fisher Scientific |
Cat # 02-671-55A |
WPI Iris scissors, 10 cm, supercut, straight |
Fisher Scientific |
Cat # 50-822-291 |
Micro dissecting forceps serrated, half curved 1 mm tip, 4 inch |
George Tiemann |
Cat # 160-18 |
Syringe filters, sterile |
Fisher Scientific |
Cat # 09720004 |
70 μm nylon mesh |
Fisher Scientific |
Cat # NC0446099 |
|
Deposited data |
|
RNA sequencing data |
Ludwik et al. 2020 |
GEO:GSE113323
|