| Minimal effort Total digestion time:2–3h |
Labor intensive Total digestion time: 1–1.5h |
| Every 20–30 min remove tube from incubator. | Every 15 min remove tube from incubator. |
| Resuspend the tissue in the digestion mixture by gentle swirling of the tube. | Pipet each tube 10–15 times using a 10 mL serological pipet |
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