TABLE 1.
Tomography resultsa
| Imaging and species or strain | Condition or strain | No. of lamellae | No. of tomograms | Source |
|---|---|---|---|---|
| Whole-cell tomography | ||||
| G. hansenii ATCC 23769 | Untreated; 13 min postseparation | NA | 6 | ATCC |
| Untreated; 20 min postseparation | NA | 14 | ||
| Untreated; 300 min postseparation | NA | 33 (24 VPP) | ||
| Cellulase, 0.2 g/liter | NA | 4 | ||
| G. xylinus ATCC 700178 | Untreated; 5 h postseparation | NA | 8 | ATCC |
| E. coli 1094 | Non-cellulose induced | NA | 1 | Gift from Jean Marc Ghigo (Institute Pasteur) |
| A. tumefaciens C58b | WT | NA | 47 | Gift from Patricia Zambrisky (UC Berkeley) to E. I. Tocheva |
| A139 | NA | 10 | Gift from Patricia Zambrisky (UC Berkeley) to E. I. Tocheva | |
| AD348 | NA | 1 | Gift from Anath Das (University of Minnesota) to D. Ghosal | |
| AD1484 | NA | 1 | Gift from Anath Das (University of Minnesota) to D. Ghosal | |
| JX148 | NA | 4 | Gift from Patricia Zambrisky (UC Berkeley) to E. I. Tocheva | |
| NT1 | NA | 2 | Gift from Patricia Zambrisky (UC Berkeley) to E. I. Tocheva | |
| Tomography on milled lamellae | ||||
| G. hansenii ATCC 23769 | Native biofilm; untreated | 10 | 19 (3 VPP) | ATCC |
| E. coli 1094 induced forcellulose synthesis | Untreated | 2 | 6 | Gift from Jean Marc Ghigo (Institut Pasteur) |
| Cellulase, 0.2 g/liter | 1 | 2 | ||
a
VPP, number of the tomograms in which the Volta phase plate was used; NA, nonapplicable; UC, University of California.
b
Strains were imaged for other purposes but used here as well. C58 (ATCC 33970) is wild-type A. tumefaciens. All A. tumefaciens strains listed had a C58 background.