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. 2021 Jan 12;15:125–140. doi: 10.2147/DDDT.S269901

Figure 5.

Figure 5

FBI-1 silencing enhances the capsaicin-induced anti-proliferation and pro-apoptosis effects in breast cancer cells. (A–C) Cells were transfected with recombinant FBI-1-silencing lentiviral particles or corresponding negative control vectors, respectively. The efficiency of FBI-1 silencing was assessed by inverted fluorescence microscopy, qRT-PCR, and Western blot. Scale bar 100 μm, or data are presented as means ± SD, *p<0.05 vs Control. (D) Cells were treated with capsaicin alone (150 μmol/L) or together with FBI-1 silencing for 72 h. The cell viability was detected by CCK-8 assay. Data are presented as means ± SD, *p<0.05 vs Control; &p<0.05 vs Capsaicin; #p<0.05 vs shRNA-FBI-1. (E) Cells were treated with capsaicin alone (150 μmol/L) or together with FBI-1 silencing for 72 h. The cell morphological changes were observed using an inverted microscope (scale bar 100 μm). (F) Cells were treated with capsaicin alone (150 μmol/L) or together with FBI-1 silencing for 72 h. The cell apoptosis rate was detected by flow cytometry. Data are presented as means ± SD, *p<0.05 vs Control; &p<0.05 vs Capsaicin; #p<0.05 vs shRNA-FBI-1.