Figure 4.

Physalis alkekengi var. franchetii extracts suppressed STAT3-mediated downstream genes in NCI-H1975 and U266 cells. (A) NCI-H1975 cells were incubated with various concentrations of the physalins for 24 h. The cell lysates were isolated for Western blot analysis to detect the Bcl-2 family, XIAP and survivin protein levels. β-actin was used as a loading control. (B) U266 cells were treated with physalins in a dose-dependent manner for 24 h. Western blot was performed with the anti-Bcl-2 family, anti-XIAP and anti-survivin primary antibodies. The semiquantification of protein levels was performed with ImageJ software. The relative gray values of Bcl-2 and XIAP are shown below.