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RT-qPCR analysis for C. burnetii-specific DNA in seropositive samples. The seropositive samples were subjected to RT-PCR analysis and the ratios were calculated from positive samples of ELISA.
| Species | No of +ve samples | Positive samples by PCR | |
|---|---|---|---|
| No. | % | ||
| Cattle | 38 | 12 | 31.6 |
| Sheep | 30 | 14 | 46.7 |
| Goats | 18 | 8 | 44.4 |
| Human | 66 | 45 | 68.2 |
| Total | 152 | 79 | 51.9 |
ELISA=Enzyme-linked immunosorbent assay, RT-qPCR=Real-time quantitative polymerase chain reaction, C. burnetii=Coxiella burnetii
