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Polymerase chain reaction setting program of amplification.
| Number of cycles | Adjusted temperature (°C) | Time | Discretion |
|---|---|---|---|
| 1 | 95 | 5 min | Initial DNA denaturation |
| 30 | 95 | 20 s | DNA denaturation |
| 57 | 30 s | Primer annealing | |
| 72 | 30 s | Primer extension | |
| 1 | 72 | 5 min | Final extension |
| 1 | 4 | Hold | Cooling |
