Table 2.

Genetic variation of the 23 polymorphic microsatellite loci in 72 accessions of A. tsao-ko

Locus	Na	Ne	Ho	He	PIC	F	FNA	HWE
AM1	4	2.444	0.514	0.591	0.516	0.130	0.073	0.233ns
AM4	3	2.461	0.479	0.594	0.533	0.193*	0.129	0.046*
AM6	3	2.056	0.514	0.514	0.395	−0.001	0.013	0.000***
AM7	4	1.877	0.472	0.467	0.413	−0.011	0.039	0.961ns
AM8	3	1.650	0.366	0.394	0.359	0.070	−0.022	0.006**
AM9	5	2.555	0.571	0.609	0.580	0.061	0.038	0.972ns
AM12	4	1.403	0.211	0.287	0.302	0.265*	0.139	0.000***
AM13	3	2.062	0.375	0.515	0.454	0.272*	−0.033	0.000***
AM17	4	1.864	0.493	0.463	0.399	−0.064	0.154	0.202ns
AM27	3	2.223	0.606	0.550	0.479	−0.101	−0.051	0.401ns
AM29	4	1.704	0.394	0.413	0.387	0.045	0.041	0.860ns
AM36	4	1.706	0.431	0.414	0.387	−0.040	0.124	0.220ns
AM40	4	2.741	0.486	0.635	0.571	0.235*	0.060	0.153ns
AM41	5	1.810	0.403	0.447	0.415	0.100	−0.096	0.916ns
AM42	4	2.939	0.779	0.660	0.655	−0.181	0.080	0.000***
AM43	4	1.315	0.208	0.239	0.228	0.129	0.107	0.010*
AM44	4	2.598	0.486	0.615	0.543	0.210*	0.008	0.001**
AM45	5	2.377	0.556	0.579	0.515	0.041	0.079	0.001**
AM49	6	1.942	0.414	0.485	0.460	0.146	0.068	0.000***
AM65	2	1.591	0.324	0.371	0.323	0.128	−0.022	0.281ns
AM89	6	3.776	0.771	0.735	0.709	−0.049	0.058	0.673ns
AM94	3	1.584	0.333	0.369	0.328	0.096	−0.009	0.342ns
AM99	3	2.267	0.569	0.559	0.492	−0.019	−0.025	0.979ns
Mean	3.913	2.305	0.468	0.500	0.454	0.072

Na, number of alleles; Ne, effective number of alleles; Ho, observed heterozygosity; He, expected heterozygosity; PIC, polymorphism information content; F, fixation index, *significance value from 95% confidence interval; FNA, frequency of null alleles; HWE deviation from Hardy–Weinberg equilibrium (ns, no significant, *p < 0.05, **p < 0.01, ***p < 0.001)