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Results of the acceptor substrate specificity studies for the α1–4-galactosyltransferase activity of NmSiaDW using sialyltrisaccharides as potential acceptors and UDP-Gal as the donor.
| Substrates | Percentage conversion (%) | ||
|---|---|---|---|
| 20 μg/mL, 10 min | 1.2 mg/mL, 10 h | ||
| 1 | S3 | 22±1 | Quant. |
| 2 | 7N3-S3 | 11±1 | Quant. |
| 3 | 9N3-S3 | 0 | Quant. |
| 4 | 7NAc-S3 | 0 | 0 |
| 5 | 9NAc-S3 | 0 | 68±3 |
