Skip to main content
. 2020 Dec 18;20(1):e13289. doi: 10.1111/acel.13289

Figure 6.

Figure 6

PBM inhibits phosphorylation of PSD‐95 and AMPA receptor endocytosis, thereby alleviating synaptic dysfunction. (a, b) Representative images (a) and quantitation (b) of total endocytosis of AMPARs (GluR1 internalized) in primary neurons derived from APP/PS1 mice under treatment with or without PBM (five individual experiments, mean ± SEM, two‐way ANOVA, **p < 0.01 vs. control group, ##p < 0.01 vs. indicated group). Scale bar: 20 μm. (c) Representative images of total endocytosis of AMPARs (GluR1 internalized) in response to different treatments. Scale bar: 20 μm. (d) Quantitation of total endocytosis of AMPARs stimulated with various treatments in primary neurons (at least three individual experiments, mean ± SEM, one‐way ANOVA, **p < 0.01 vs. control group, #p < 0.05 vs. indicated group). (e, g) Representative Western blot analysis was performed to detect p‐AMPAR, and AMPAR stimulated with Aβ1‐42 and/or PBM in the presence of PD98059 in primary neurons. Immunoprecipitates were analyzed for detecting p‐PSD95, and PSD95 stimulated with various treatments in primary neurons (at least three individual experiments, mean ± SEM, one‐way ANOVA, *p < 0.05 vs. control group, #p < 0.05 vs. indicated group, ##p < 0.01 vs. indicated group). (f, h) Representative Western blot assay of p‐AMPAR, and AMPAR, and immunoprecipitation of p‐PSD95 and PSD95 in neurons derived from APP/PS1 mice under treatment with or without PBM (at least three individual experiments, mean ± SEM, two‐way ANOVA, **p < 0.01 vs. control group). (i, j) Representative immunoblots of PSD proteins from APP/PS1 mice treated with or without PBM (at least three individual experiments, mean ± SEM, two‐way ANOVA, **p < 0.01 vs. control group). (k) Representative Western blots of PSD proteins from cultured neurons in response to different treatments. (l) Quantification of (k). Data are presented as means ± SEM for at least three individual experiments. **p < 0.01 vs. control group; ##p < 0.01 vs. indicated group. Please refer to Figure S10 to see that inhibition of JNK3 can alleviate AMPA receptor endocytosis