Table 2.
Top pathways ‐ negative mode
| Sham_Old versus Sham_Young | ||||
|---|---|---|---|---|
| Pathways | Overlap_size | Pathway_size | p‐value (raw) | p‐value |
| tRNA charging pathway | 5 | 8 | 0.55167 | 0.01285 |
| sucrose degradation V (mammalian) | 3 | 4 | 0.44072 | 0.01328 |
| guanine and guanosine salvage II | 2 | 2 | 0.33516 | 0.0188 |
| adenine and adenosine salvage III | 2 | 2 | 0.33516 | 0.0188 |
| S‐adenosyl‐L‐methionine cycle II | 2 | 2 | 0.33516 | 0.0188 |
| lipoate biosynthesis and incorporation I | 2 | 2 | 0.33516 | 0.0188 |
| lipoate biosynthesis and incorporation II | 2 | 2 | 0.33516 | 0.0188 |
| glycine betaine degradation | 2 | 2 | 0.33516 | 0.0188 |
| arsenate detoxification I (glutaredoxin) | 2 | 2 | 0.33516 | 0.0188 |
| sucrose degradation | 2 | 2 | 0.33516 | 0.0188 |
| galactose degradation I (Leloir pathway) | 2 | 2 | 0.33516 | 0.0188 |
| lactose degradation III | 2 | 2 | 0.33516 | 0.0188 |
| melibiose degradation | 2 | 2 | 0.33516 | 0.0188 |
| glucose and glucose−1‐phosphate degradation | 2 | 2 | 0.33516 | 0.0188 |
| methionine degradation I (to homocysteine) | 2 | 2 | 0.33516 | 0.0188 |
| I/R_Old versus I/R_Young | ||||
|---|---|---|---|---|
| Pathways | overlap_size | pathway_size | p‐value (raw) | p‐value |
| bile acid biosynthesis, neutral pathway | 3 | 4 | 0.25705 | 0.00542 |
| adenine and adenosine salvage III | 2 | 2 | 0.21341 | 0.01468 |
| glucose and glucose−1‐phosphate degradation | 2 | 2 | 0.21341 | 0.01468 |
| L‐ascorbate biosynthesis VI | 2 | 3 | 0.44714 | 0.07683 |
| adenosine nucleotides degradation II | 2 | 3 | 0.44714 | 0.07683 |
Enrichment of pathways was tested using Mummichog software. Differences between “Sham_Old vs Sham_Young” and “I/R_Old vs I/R_Young” in negative mode have been shown. An adjusted p‐value per pathway is calculated based on the EASE score and cumulative distribution function (Li et al., 2013).