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. 2020 Oct 5;37(1):15–30. doi: 10.1007/s12264-020-00583-7

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Knockdown of Foxg1 decreases proliferation while promoting the differentiation of OPCs in vitro. A Foxg1 knockdown decreases the expression of Foxg1 in primary cultured rat OPCs. Cells transfected with a lentiviral GFP-tagged vector inserted into a scrambled sequence (Control) or lentiviral GFP-tagged Foxg1 shRNA (shRNA) for 3 days and immunostained with anti-Foxg1 antibody. B Quantification of Foxg1 fluorescence integrated optical density (IOD) as in A. C The transfection efficiency as defined by GFP fluorescence. D Western blots and quantitative analyses of Foxg1 protein expression in primary cultured rat OPCs. E, F Representative images of EdU-positive proliferating cells (E) and quantitative analysis (F) of Control and Foxg1 shRNA-treated OPCs. G–I Representative images of NG2 (G), O4 (H), and CNPase (I) staining in each group of cells. J Cell proportions at different stages during the differentiation of OPCs. White arrows, representative double-labeled positive cells; n = 6 per group; data are presented as the mean ± SEM; *P < 0.05, ***P < 0.001, n.s. not significant, Student’s t-test; scale bars, 40 μm.