Fig. 1.

Phylogeny of FirmiCODHech genes and targets of new primers. The tree was constructed using an alignment of 1558 CODH proteins. Major clades B through F are indicated on the right. FirmiCODHech subclades are assigned and colored in red. The colors of the branches and leaves indicate the following: red, FirmiCODHechs; black, other CODHs. The targets of each new primer set are shown by squares placed to the right of the leaves in different colors. Asterisks indicate FirmiCODHech genes added in the CODHech-mock community sample. The black squares on the branch indicate > 0.8 support by bootstrap values. The top right box shows a schematic representation of amplification regions of each primer set