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Validation of TM4SF4 as an outlier gene. (A) Representative images for immunohistochemistry of TM4SF4-low (others) and TM4SF4-high (outlier) lung adenocarcinoma. (B–D) TM4SF4 expression is validated using quantitative reverse transcription–polymerase chain reaction, flow cytometry (fluorescence-activated cell sorting), and immunohistochemistry in lung adenocarcinoma cell lines.
