Fig. 5.

Validation of LRRK2 as an outlier gene. (A) Representative images for immunohistochemistry of LRRK2 expression. In total, 552 breast cancer samples are investigated. (B, C) LRRK2 expression is validated by quantitative real-time polymerase chain reaction and immunoblotting in breast cancer cell lines. The blots crop from different parts of the same gel. The values below the gels represent the LRRK2 protein signal intensities after normalization to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) protein signal intensities.