Fig. 2.

Tracheospheres derived and mouse tracheal basal cells and grown in 3D conditions are comprised of multiple airway cell types. a Brightfield image of live tracheospheres in 3D culture. b Immunofluorescence staining of 5 µm paraffin embedded sections from tracheospheres stained for basal marker cytokeratin 5 (green), lumenal cell marker cytokeratin 8 (red) and nuclei (blue). c Immunofluorescence staining of 5 µm paraffin embedded sections from tracheospheres stained for basal marker cytokeratin 5 (green), ciliated cell marker acetylated tubulin (red) and nuclei (blue). d Magnified image of immunofluorescence staining of 5 µm paraffin embedded sections from tracheospheres stained for basal marker cytokeratin 5 (green), ciliated cell marker acetylated tubulin (red) and nuclei (blue) as seen in (c). Scale bars are 100 µm in (a–c), 10 µm in (d)