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. 2020 Nov 18;12(2):210–217. doi: 10.1111/1759-7714.13745

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© 2020 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Overexpression of CADM1 inhibits the progression of LUAD. A549 and H1299 cells were transfected with pcDNA‐CADM1 or pcDNA negative control. (a) RT‐qPCR analysis of the transfection efficiency and CCK‐8 assay for cell viability , pc‐NC; , pc‐CADM1; , pc‐NC; , pc‐CADM1; , pc‐NC; , pc‐CADM1. (b) EdU assay for cell proliferation , pc‐NC; , pc‐CADM1; (c) Cell capacity of migration (scratch test) , pc‐NC; , pc‐CADM1. (d) Transwell assay for cell migration , pc‐NC; , pc‐CADM1; (e) Transwell assay for cell invasion , pc‐NC; , pc‐CADM1; (f) Western blot assay of the expression level of migration related proteins (Cox‐2, MMP‐2 and MMP‐9) , pc‐NC; , pc‐CADM1; , pc‐NC; , pc‐CADM1; **P < 0.01 versus pc‐NC group.

Inline graphic — Overexpression of CADM1 inhibits the progression of LUAD. A549 and H1299 cells were transfected with pcDNA‐CADM1 or pcDNA negative control. (a) RT‐qPCR analysis of the transfection efficiency and CCK‐8 assay for cell viability , pc‐NC; , pc‐CADM1; , pc‐NC; , pc‐CADM1; , pc‐NC; , pc‐CADM1. (b) EdU assay for cell proliferation , pc‐NC; , pc‐CADM1; (c) Cell capacity of migration (scratch test) , pc‐NC; , pc‐CADM1. (d) Transwell assay for cell migration , pc‐NC; , pc‐CADM1; (e) Transwell assay for cell invasion , pc‐NC; , pc‐CADM1; (f) Western blot assay of the expression level of migration related proteins (Cox‐2, MMP‐2 and MMP‐9) , pc‐NC; , pc‐CADM1; , pc‐NC; , pc‐CADM1; **P < 0.01 versus pc‐NC group.