FIGURE 4.

Optical clearing and imaging of diverse tissue clearing techniques. (a) Organic solvent‐based tissue clearing and volumetric imaging. Images adapted from Refs (Erturk et al., 2012; Pan et al., 2016; Jing et al., 2018; Cai et al., 2019). (b) Aqueous‐based tissue clearing and optical imaging. Images adapted from Refs (Ke et al., 2013; Kuwajima et al., 2013; Hou et al., 2015; Ke et al., 2016; Li et al., 2017; Xu et al., 2017; Tainaka et al., 2018). (c) Hydrogel embedding tissue clearing and 3D imaging. Images adapted from Refs (Chung et al., 2013; Yang et al., 2014; Kim et al., 2015; Murray et al., 2015; Park et al., 2019)