TABLE 1.

Common procedures of tissue clearing

Procedure	Reagent	Method	References
Tissue fixation	PFA	3DISCO	Erturk et al., 2012
	GA	SWITCH	Murray et al., 2015
	Acrylamide hydrogel	CLARITY	Chung et al., 2013
Permeabilization
Solvents	Ethanol	BABB	Dodt et al., 2007
	THF, DCM	FDISCO	Qi et al., 2019
	Tert‐butanol	uDISCO	Pan et al., 2016
Hydration	Urea	Scale	Hama et al., 2011
Delipidation	Aminoalcohols	CUBIC	Susaki et al., 2014
	SDS	FACT	Xu et al., 2017
	Saponin	SeeDB2	Ke et al., 2016
	Triton X−100	Ce3D	Li et al., 2017
Decolorization	H2O2	iDISCO	Renier et al., 2014
	Quadrol	PEGASOS	Jing et al., 2018
	Ammonium	CUBIC	Tainaka et al., 2014
	N‐alkylimidazole	CUBIC‐L/R+	Tainaka et al., 2018
	Thioglycerol	FRUIT	Hou et al., 2015
RI matching	DBE	3DISCO	Erturk et al., 2012
	DPE	uDISCO	Pan et al., 2016
	PEG	PEGASOS	Jing et al., 2018
	Sucrose	SeeDB	Ke et al., 2013
	Aromatic amide	CUBIC‐L/R+	Tainaka et al., 2018
	FocusClear	CLARITY	Chung et al., 2013
	RIMS	PACT‐PARS	Yang et al., 2014
	Iohexol	SeeDB2	Ke et al., 2016

Abbreviations: BABB, benzyl alcohol and benzyl benzoate; DBE, dibenzyl ether; DCM, dichloromethane; DPE, diphenyl ether; GA, glutaraldehyde; PEG, polyethylene glycol; PFA, paraformaldehyde; SDS, sodium dodecyl sulfate; THF, tetrahydrofuran.