TABLE 3.

Comparison of aqueous‐based tissue clearing

Method	Clearing capability	Tissue size	DiI Compatible	TEM Compatible	Tissue validation	References
Simple immersion
SeeDB	Weak	Constant	Yes	yes	Mouse hemi‐brain clearing	Ke et al., 2013
FRUIT	Medium	Constant	Yes	N.T.	Whole adult rabbit Brain clearing	Hou et al., 2015
ClearT/T2	Weak	Constant	Yes	N.T.	Young mouse brain clearing	Kuwajima et al., 2013
RTF	Medium	Constant	Yes	N.T.	Adult mouse brain blocks clearing	Yu et al., 2018
Delipidation
FACT	Strong	Minor shrinkage	No	No	Whole adult mouse brain clearing	Xu et al., 2017
SeeDB2	Medium	Constant	Yes	No	Mouse hemi‐brain clearing	Ke et al., 2016
FOCM	Strong	Constant	yes	N.T.	Mouse hemi‐brain clearing	Zhu et al., 2019
Ce3D	Strong	Minor shrinkage	N.T.	N.T.	Whole lymph node clearing	Li et al., 2017
Hydration/delipidation
Scale	weak	expansion	N.T.	yes	Adult mouse brain section clearing	Hama et al., 2011
ScaleS	Strong	Transient expansion	yes	yes	Mouse hemi‐brain clearing	Hama et al., 2015
CUBIC	Strong	Expansion	no	no	Whole adult mouse Brain clearing	Susaki et al., 2014
Updated CUBIC	Very strong	Expansion	no	no	Whole adult mouse Body clearing	Tainaka et al., 2018
UbasM	Strong	Expansion	yes	N.T.	Mouse hemi‐brain clearing	Chen et al., 2017

Abbreviations: N.T., not tested; TEM, transmission electron microscope.