TABLE 4.
Comparison of hydrogel embedding tissue clearing
| Method | Electrophoretic driven | Tissue validation | Clearing rate | Immunostaining | Antigenicity preservation | TEM | References |
|---|---|---|---|---|---|---|---|
| CLARITY | Yes | Whole mouse brain | Fast | Yes | Good | No | Chung et al., 2013 |
| ACT‐PRESTO | Yes | Mouse and rabbit brain | Fast | Yes | Good | No | Lee et al., 2016 |
| PRE‐CLARITY | Yes | Whole mouse brain | Fast | Yes | Good | No | Du et al., 2019 |
| Stochastic electrotransport | Yes | Whole mouse brain | Very fast | Yes | Good | No | Kim et al., 2015 |
| passive CLARITY | No | Whole mouse brain | Slow | Yes | Good | No | Tomer et al., 2014 |
| PACT‐PARS | No | Whole mouse body | Fast | Yes | Good | No | Yang et al., 2014 |
| bone CLARITY | No | Bones of trunk and limbs | Fast | Yes | Good | No | Greenbaum et al., 2017 |
| SWITCH | No | Rat brain human sample | Very fast | Yes | Excellent | No | Murray et al., 2015 |
| SHIELD | No | Mouse brain human block | Very fast | Yes | Excellent | No | Park et al., 2019 |