Schematic graphic comparing the MPN efferences between the control (a) and OVX (b) groups. A decrease in the density of the projections to regions related to female sexual behaviour in the OVX group compared to the control group was observed (arrows in grey in a and dotted arrows in b). ARH, arcuate nucleus of the hypothalamus; AVP, preoptic anteroventral nuclei; AVPV, anteroventral periventricular nucleus; BNST, bed nucleus of the stria terminalis; MeApd, medial nucleus of the amygdala, posterodorsal division; MPN, medial preoptic nucleus; PAGvl, periaqueductal grey matter, ventrolateral division; PMv, ventral premammillary nuclei; VMHvl, ventromedial nucleus of the hypothalamus, ventrolateral division; VTA, ventral tegmental area