Figure 4.

Effects of TNF on the suppressive function of TNFR2⁺Tregs. Purified CD4⁺ T cells were cultured in medium alone, TNF (50 ng/mL), TNF combined with anti-TNFR2 mAbs (10 µg/mL), or isotype IgG, as indicated, for 72 hours. The representative FACS analysis of (A) CTLA-4⁺ cells and (B) PD-L1⁺ cells in TNFR2⁺ Tregs as indicated, gated on live CD4⁺Foxp3⁺ cells. Summary of the proportions of (C) CTLA-4⁺ cells (n=3) and (D) PD-L1⁺ cells (n=3) in Tregs within each condition. Data are expressed as means ± SEM. *, P<0.05, **, P<0.01, ****, P<0.0001 by one-way ANOVA test. NS represents no statistics difference. TNFR2, tumor necrosis factor receptor type II.