Table 1. The sources of the primary and secondary antibodies.
Cells or molecule | Primary antibody (source) | Secondary antibody (source) |
---|---|---|
ApoM | Rabbit anti-human apoM (GeneTex) | PE-goat anti-rabbit (Proteintech) |
T cell | FITC-mouse anti-human CD3 (BD) | – |
Monocyte | FITC-mouse anti-human CD14 (BD) | – |
NK cell | PE-Cy5-mouse anti-human CD56 (BD) | – |
NK cell | APC-mouse anti-human CD16 (BioLegend) | – |
B cell | PerCP-mouse anti-human CD19 (BioLegend) | – |
Isotype control | Rabbit IgG (Abcam) | – |
ApoM, apolipoprotein M; NK cell, natural killer cell; CD, cluster of differentiation; FITC, fluorescein isothiocyanate; PE-Cy5, phycoerythrin-Cy5 conjugate; APC, allophycocyanin; PerCP, peridinin-chlorophyll-proteincomplex; PE, R-Phycoerythrin.