Table 2. Groupings and corresponding conjugated fluorochromes.
| Grouping | Added antibody |
|---|---|
| 1 | Cell only |
| 2 | FITC-CD3 |
| 3 | PerCP-CD19 |
| 4 | FITC-CD14 |
| 5 | PE-Cy5-CD56 |
| 6 | APC-CD16 |
| 7 | ApoM-Ab and PE-goat anti-rabbit |
| 8 | ApoM-Ab, PE-goat anti-rabbit, and FITC-CD3 |
| 9 | ApoM-Ab, PE-goat anti-rabbit, and PerCP-CD19 |
| 10 | ApoM-Ab, PE-goat anti-rabbit, and FITC-CD14 |
| 11 | ApoM-Ab, PE-goat anti-rabbit, and PE-Cy5-CD56 |
| 12 | ApoM-Ab, PE-goat anti-rabbit, and APC-CD16 |
| 13 | ApoM-Ab, PE-goat anti-rabbit, and FITC-CD3 and PerCP-CD19 |
| 14 | ApoM-Ab, PE-goat anti-rabbit, FITC-CD3, APC-CD16, and PE-Cy5-CD56 |
| 15 | Isotype control and PE-goat anti-rabbit |
ApoM, apolipoprotein M; NK cell, natural killer cell; CD, cluster of differentiation; FITC, fluorescein isothiocyanate; PE-Cy5, phycoerythrin-Cy5 conjugate; APC, allophycocyanin; PerCP, peridinin-chlorophyll-proteincomplex; PE, R-Phycoerythrin.