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. 2020 Dec;8(24):1678. doi: 10.21037/atm-20-7939

Figure 2.

Figure 2

Establishment of a lung cancer cell line that interferes with SALL4. (A) RT-PCR detection of SALL4 expression in five lung cancer cell lines, A549, H157, H1650, H358, and H1703, and normal 16-HBE bronchial epithelial cells. (B) Western blotting detection of SALL4 expression in lung cancer cell lines and normal bronchial epithelial cells. (C) RT-PCR detection of the effect of siRNA-SALL4 transfection on SALL4 mRNA expression in A549 and H358 cells. (D) Western blotting detection of transfection siRNA and the effect of SALL4 on the expression of SALL4 in A549 and H358 cells. *, P<0.05. SALL4, Sal-like protein 4; RT-PCR, reverse transcription-polymerase chain reaction; mRNA, messenger RNA.