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Effect of neferine on melanoma cell proliferation. (A,B) 5-ethynyl-2’-deoxyuridine (EdU) staining of A375 and C32 cells. (C,D) Cell viability of A375 and C32 cells exposed to 0–72 h were detected by cell counting Kit-8 assay. (E,F,G) Relative protein levels of Ki67 and survivin were detected by Western blot in A375 and C32 cells. Data are presented as mean ± standard deviation. *, P<0.05, **, P<0.01 vs. control. DAPI, 4’,6’-diamidino-2-phenylindole dihydrochloride.
