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. 2020 Dec;8(24):1643. doi: 10.21037/atm-20-7201

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2020 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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Effect of Neferine on oxidative stress of melanoma cells. (A) Reactive oxygen species (ROS) production was detected by 2,7-dichlorodi-hydrofluorescein diacetate probes. Superoxide dismutase (SOD) level (B) and 3,4-methylenedioxyamphetamine (MDA) content (C) were detected by enzyme-linked immunosorbent assay. (D) Reactive oxygen species (ROS) production was detected by 2,7-dichlorodi-hydrofluorescein diacetate probes. (E) Relative protein levels of cleaved-caspase (Cas)-3 and cleaved-Cas-9 were detected by western blot. Data are presented as mean ± standard deviation. *, P<0.05, **, P<0.01 vs. control. ^#, P<0.05 vs. NAC. NAC, N-acetylcysteine.