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. 2020 Dec;8(24):1643. doi: 10.21037/atm-20-7201

Figure 6.

Figure 6

Neferine activates the p38 mitogen-activated protein kinase/Jun-N-terminal kinase (JNK)1/2 pathway. (A,B,C) Expression of p38, p-p38, JNK1/2 and p-JNK1/2 was determined by western blot in A375 and C32 cells. After adding p38 inhibitor SB203580, the expression of p38, p-p38, JNK1/2, and p-JNK1/2 was determined by western blot in A375 cells (D). Superoxide dismutase (SOD) level (E) and 3,4-methylenedioxyamphetamine (MDA) content (F) were detected by enzyme-linked immunosorbent assay in A375 cells. (G,I) 5-Ethynyl-2’-deoxyuridine (EdU) staining of A375 cells. (H,J) Apoptotic ratio of A375 cells. Data are presented as mean ± standard deviation. *, P<0.05, **, P<0.01 vs. control. #, P<0.05 vs. SB203580. DAPI, 4’,6’-diamidino-2-phenylindole dihydrochloride.