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. 2020 Dec;8(24):1651. doi: 10.21037/atm-20-7703

Figure 4.

Figure 4

Determination of targeting relationship between miR-146b-5p and Robo1. (A) Predicted binding sites betweenRobo1 and miR-146b-5p. (B) The mRNA expressive levels of miR-146b-5p in 293T cells were detected by RT-qPCR. (C) 293T cells were co-transfected with Robo1-Wt or Robo1-Mut and miR-146b-5p mimic, followed by the measurement of luciferase activity. (D) The mRNA expressive levels of Robo1 in 293T cells was detected by RT-qPCR. (E) The relative protein levels of Robo1were detected by western blot in the control group, miR-146b-5p mimic group, Robo1 group, miR-146b-5p mimic + Robo1 group. (F) The relative protein levels of Robo1 were detected by western blot. *, P<0.05, **, P<0.01, ***, P<0.001, compared with control group; #, P<0.05, compared with ALI model group; &, P<0.05, compared with ALI model group of injection miR-146b-5p antagomir; ##, P<0.01, compared with Ad-Robo1. Robo1, Roundabout 1.