FIGURE 4.

Effects of Sal B on HG‐induced podocytes ROS generation and apoptosis. Human podocytes were exposed either to HG (35 mM) and with or without the Sal B (200 µM), GKT137831 (20 µM) or to H₂O₂ (200 µM) in NG(5 mM) for 24 h: A(i), podocytes apoptosis examined by flow cytometry assay A(ii), quantification of per cent annexin V‐positive cells. B(i), ROS measurement using 2,7‐dichlorofluorescein (DCF). B(ii), statistical analysis of ROS fluorescence intensity (MFI) values. C(i), representative western blot of Nox4 levels in podocytes. C(ii), western blots from three experiments were quantified by densitometry analysis. Values are presented as the means ± SEM (^* P < .05, ^*** P < .001 vs NG group; ^# P < .05 vs HG group)