FIGURE 5.

Sal B alleviated HG‐induced NOX4 up‐regulation part through AMPK regulation. A(i), representative western blot of p‐AMPKα^Thr172A,AMPKα, NOX4. A(ii), histogram representing AMPK activity measured in podocytes treated in NG (5 mM) or HG (35 mM) and in the presence or absence of Sal B (50 µM, 200 µM) for 24 h. B(i), In parallel experiments, podocytes were transfected with siNC in NG or transfected with siRNA‐AMPKα in HG and in the presence or absence of Sal B (200 µM) for 24 h. Shown is a representative western blot of p‐AMPKα^Thr172A, AMPKα, NOX4.B(ii): histogram representing AMPK and NOX4 activity measured as indicated. C, ROS measurement using 2,7‐dichlorofluorescein (DCF) D, MitoSOX (red) immunofluorescence in podocytes cultured as described in B. The results were from three independent experiments expressed as the means ± SEM (^* P < .01, ^** P < .01, ^*** P < .001 vs NG group)