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. 2020 Dec 17;25(2):1012–1023. doi: 10.1111/jcmm.16165

FIGURE 6.

FIGURE 6

Effect of Sal B on oxidative stress mediated podocyte apoptosis is associated with down‐regulation of NOX4. A(i), Effect of Nox4 siRNA and siNC (24 h) on Nox4 protein expression in podocytes. A(ii), Relative mRNA amount of NOX4. B(i), Apoptosis of podocytes intervened by siNOX4 detected by flow cytometry using Annexin V‐PI staining B(ii), Quantification of per cent annexin V‐positive cells. C(i), Histogram representing ROS fluorescence intensity (MFI)values in podocytes transfected with siNC or siRNA‐NOX4 in HG or NG and in the presence or absence of Sal B (200 uM) for 24h. C(ii), Histogram representing MitoSOX fluorescence intensity (MFI) values in podocytes cultured as described in C(i). The results were from three independent experiments expressed as the means ± SEM (*P < .05; **P < .01; ***P < .001 vs NG group)