FIGURE 1.

Gracillin inhibits proliferation and migration of human CRC cells. A, Chemical structure of gracillin. B, RKO, HCT116 and SW480 cells treated with increasing concentrations of gracillin for 48 h. Cells’ viability was evaluated by the MTT assay, and the half‐maximal inhibitory concentration (IC₅₀) was calculated using the GraphPad Prism software. Data are presented as means of three experiments, and error bars represent SD. C, HCT116, SW480 and RKO cells were incubated with different concentrations of gracillin (0, 2.5, 5.0 or 10.0 μmol/L) for 24 h. Colonies were fixed, and micrographs were acquired. D, RKO cell cultures, grown to 80%‐90% confluence, were scratched to form 200 µm‐wide wounds. Cells were then treated with gracillin at different concentrations (0, 1.0, 2.5 or 5.0 μmol/L) for 24 h. Micrographs were acquired at 0 h and 24 h after treatment with gracillin